A549 Transfection Reagent
| Catalog # 1705 - 0.5 mL
|
$ 170 |
 |
| Catalog # 1706 - 1.5 mL
|
$ 305 |
|
Transfection reagent for A549 cells
- Kit includes Transfection Enhancer reagent and recommended transfection protocols
- Efficient transfection of lung carcinoma cells
- Protocols provided for transfection of siRNA, DNA and microRNA
- High cell viability
- Work in presense of serum
- Efficient for plasmid DNA/siRNA cotransfection
- Reproducible transfection
- Suitable for Reverse Transfection
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by Fluorescent microscopy, real-time RT-PCR, Western blot.
Transfection Protocol:
Transfection protocols are provided with the purchase of the reagent.
Figure 1. SiRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=3).
