NIH3T3 Transfection Reagent
| Catalog # 3211 - 0.5 mL
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$ 115 |
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| Catalog # 3212 - 1.5 mL
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$ 271 |
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 Transfection reagent for NIH3T3 cells
- Kit includes Transfection Enhancer reagent and recommended transfection protocols
- Protocols provided for transfection of mRNA, siRNA and microRNA
- High transfection efficiency
- Work in presence of serum
- Compatible with transfection in any plate format
- Reproducible transfection
- High cell viability
- Optimized transfection protocols are adapted for use with both standard & reverse transfection methods
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 85% transfection efficiency of siRNA delivery. Transfection efficiency was determined by real-time RT-PCR.
Transfection Protocol:
Transfection protocols are provided with the purchase of the reagent.
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).
 
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